NPDC-1 CRISPR/Cas9 KO Plasmid (m): sc-421942
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NPDC-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NPDC-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NPDC-1 CRISPR/Cas9 KO Plasmid (m) | sc-421942 | 20 µg | $397.00 |
Overview
Npdc1 encodes NPDC-1, a neuronal and glial differentiation-associated protein implicated in the control of cell cycle progression and maturation programs within the nervous system. NPDC-1 has been linked to regulation of proliferation-to-differentiation transitions, with reported interactions that influence transcriptional outputs and growth control pathways in neural lineages. In mouse models and neural cell systems, altered NPDC-1 activity is used to interrogate mechanisms of neurite outgrowth, neuronal plasticity, and lineage specification. Dysregulation of these processes is relevant to neurodevelopmental abnormalities and to cellular states associated with aberrant proliferation in nervous tissue.
NPDC-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Npdc1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Npdc1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Npdc1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NPDC-1 protein expression.
This CRISPR knockout system enables efficient generation of Npdc1-deficient cell models for investigation of NPDC-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Npdc1 exon(s) critical for NPDC-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Npdc1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NPDC-1 CRISPR/Cas9 KO Plasmid (m) and NPDC-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Npdc1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NPDC-1 HDR Plasmid (m) and NPDC-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Npdc1 homology arms to support homology-directed repair at defined Npdc1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.