Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NP1 CRISPR/Cas9 KO Plasmid (m): sc-421951
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NP1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NP1 Antibody (B-8): sc-374199
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NP1 CRISPR/Cas9 KO Plasmid (m) | sc-421951 | 20 µg | $397.00 |
Overview
Neuronal pentraxin 1 (Nptx1; NP1) is a secreted neuronal pentraxin enriched at excitatory synapses, where it contributes to activity-dependent synapse remodeling and the organization of glutamatergic signaling. NP1 participates in extracellular protein networks that influence AMPA receptor clustering and synaptic stabilization, linking neuronal activity to circuit refinement and plasticity. In mouse systems, Nptx1 regulation has been associated with processes including synaptogenesis, neurite outgrowth, and calcium-dependent signaling cascades that shape experience-dependent connectivity. Altered NP1 expression and neuronal pentraxin pathway dynamics have been implicated in neurodegeneration- and seizure-related phenotypes, supporting its relevance for mechanistic studies of synaptic dysfunction.
NP1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nptx1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nptx1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nptx1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NP1 protein expression.
This CRISPR knockout system enables efficient generation of Nptx1-deficient cell models for investigation of NP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nptx1 exon(s) critical for NP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nptx1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NP1 CRISPR/Cas9 KO Plasmid (m) and NP1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nptx1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NP1 HDR Plasmid (m) and NP1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nptx1 homology arms to support homology-directed repair at defined Nptx1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.