NOXA1 Double Nickase Plasmid (h): sc-416586-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NOXA1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NOXA1 Double Nickase Plasmid (h) and NOXA1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NOXA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NOXA1 Antibody (H-6): sc-398873
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NOXA1 Double Nickase Plasmid (h) | sc-416586-NIC | 20 µg | $410.00 | |||
NOXA1 Double Nickase Plasmid (h2) | sc-416586-NIC-2 | 20 µg | $410.00 |
NOXA1 (NADPH oxidase activator 1) encodes a regulatory subunit that promotes activation of the NOX1 NADPH oxidase complex by facilitating assembly with p22phox and organizer proteins, thereby driving controlled production of reactive oxygen species (ROS). NOXA1-dependent ROS signaling contributes to redox-sensitive pathways that influence cytoskeletal remodeling, vesicle trafficking, and transcriptional programs linked to proliferation and inflammatory responses. As a modulator of oxidative signaling, NOXA1 is studied in contexts where ROS impacts epithelial barrier function and innate immune signaling, and where dysregulated redox balance contributes to cellular stress phenotypes. Altered NOX1/NOXA1 axis activity has been investigated in models of chronic inflammation and tumor-associated signaling, supporting its relevance for mechanistic research.
NOXA1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NOXA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NOXA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NOXA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NOXA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.