NOR-1 CRISPR/Cas9 KO Plasmid (h): sc-401813
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NOR-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NOR-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NOR-1 Antibody (H-7): sc-393902
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NOR-1 CRISPR/Cas9 KO Plasmid (h) | sc-401813 | 20 µg | $397.00 |
Overview
NR4A3 encodes the orphan nuclear receptor NOR-1 (NR4A3), an immediate-early, ligand-independent transcription factor that rapidly couples extracellular cues to gene expression programs. NOR-1 regulates cellular differentiation, proliferation, apoptosis, and metabolic adaptation by modulating transcriptional networks downstream of MAPK/ERK signaling and other stress-responsive pathways, and it can influence inflammatory and immune-associated gene expression. Dysregulated NR4A3 activity has been linked to oncogenic and metabolic phenotypes, and NR4A3 rearrangements are implicated in certain sarcomas, supporting its relevance for mechanistic studies of transcriptional control in disease-related contexts.
NOR-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NR4A3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NR4A3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NR4A3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NOR-1 protein expression.
This CRISPR knockout system enables efficient generation of NR4A3-deficient cell models for investigation of NOR-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NR4A3 exon(s) critical for NOR-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NR4A3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NOR-1 CRISPR/Cas9 KO Plasmid (h) and NOR-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NR4A3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NOR-1 HDR Plasmid (h) and NOR-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NR4A3 homology arms to support homology-directed repair at defined NR4A3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.