NHERF-1 CRISPR/Cas9 KO Plasmid (m): sc-424152
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NHERF-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NHERF-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NHERF-1 Antibody (A-7): sc-271552
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NHERF-1 CRISPR/Cas9 KO Plasmid (m) | sc-424152 | 20 µg | $397.00 |
Overview
Slc9a3r1 encodes NHERF-1 (Na⁺/H⁺ exchanger regulatory factor 1), a PDZ domain–containing scaffold that organizes membrane receptors, ion transporters, and signaling proteins at the apical cortex. NHERF-1 couples complexes such as NHE3 with cytoskeletal adaptors (e.g., ezrin) to coordinate epithelial ion transport, polarity, and microvillar structure, and it modulates GPCR and receptor tyrosine kinase signaling dynamics. Through these interactions, it influences processes including endocytosis, trafficking, and phosphoinositide-dependent signaling pathways that shape cellular homeostasis. Dysregulation of NHERF-1–dependent scaffolding has been linked to epithelial dysfunction and aberrant signaling states relevant to studies of inflammation, barrier integrity, and oncogenic pathway remodeling.
NHERF-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Slc9a3r1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Slc9a3r1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Slc9a3r1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NHERF-1 protein expression.
This CRISPR knockout system enables efficient generation of Slc9a3r1-deficient cell models for investigation of NHERF-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Slc9a3r1 exon(s) critical for NHERF-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Slc9a3r1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NHERF-1 CRISPR/Cas9 KO Plasmid (m) and NHERF-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Slc9a3r1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NHERF-1 HDR Plasmid (m) and NHERF-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Slc9a3r1 homology arms to support homology-directed repair at defined Slc9a3r1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.