Anticorpo NHE-9 (B-2) raccomandato per il rilevamento di NHE-9 di origine mouse, rat e human in WB, IP, IF e ELISA

Anticorpo NHE-9 (B-2): sc-515758

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Schede tecniche

    • Anti-NHE-9 Antibody B-2 è un monoclonale di topo IgG1 (kappa light chain) NHE-9 antibody fornito in 200 µg/ml
    • generato contro aminoacidi 51-131 mapping vicino il N-terminus del NHE-9 di origine human
    • NHE-9 Antibody (B-2) é raccomandato per il rilevamento di NHE-9 di origine mouse, rat e human in WB, IP, IF e ELISA
    • NHE-9 Antibody (B-2) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di NHE-9 (B-2): sc-515758.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NHE-9 Antibody (B-2) for WB applications. These reagents are now offered in bundles with NHE-9 Antibody (B-2) (see ordering information below).
loading

How much sample do you recommend loading on the gel for Western blot detection using NHE-9 (B-2): sc-515758?

Richiesta da: TinTin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Risposta da: Technical Support
Data di pubblicazione: 2017-03-01
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