NF-YC Antibody (C-2): sc-390861

NF-YC Antibody (C-2) is a mouse monoclonal IgG3 antibody that detects NF-YC in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NF-YC, a crucial component of the CCAAT-binding factor NF-Y, plays a significant role in gene regulation by binding to CCAAT sequences in the promoter regions of various eukaryotic genes, thereby influencing transcriptional activity. The NF-Y complex is composed of three subunits: NF-YA, NF-YB, and NF-YC, with all three being essential for effective DNA binding. NF-YC is located in the nucleus, where NF-YC participates in the assembly of the NF-Y complex, facilitating the transcription of genes involved in critical cellular processes such as cell cycle regulation and differentiation. The interaction between NF-YB and NF-YC subunits is particularly interesting, as these subunits engage in a protein-protein interaction through a histone-fold "handshake" motif, which is analogous to the interactions seen in histone proteins. This structural similarity underscores the evolutionary conservation of the NF-Y complex and highlights NF-YC′s importance in maintaining proper gene expression across different species. NF-YC (C-2) antibody is an invaluable tool for researchers studying transcriptional regulation and the molecular mechanisms underlying various biological processes.

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NF-YC Antibody (C-2) References:

1. NF-Y coassociates with FOS at promoters, enhancers, repetitive elements, and inactive chromatin regions, and is stereo-positioned with growth-controlling transcription factors.  |  Fleming, JD., et al. 2013. Genome Res. 23: 1195-209. PMID: 23595228
2. Nuclear transcription factor Y and its roles in cellular processes related to human disease.  |  Ly, LL., et al. 2013. Am J Cancer Res. 3: 339-46. PMID: 23977444
3. Transcriptional activation of PRMT5 by NF-Y is required for cell growth and negatively regulated by the PKC/c-Fos signaling in prostate cancer cells.  |  Zhang, HT., et al. 2014. Biochim Biophys Acta. 1839: 1330-40. PMID: 25281873
4. NF-YC in glioma cell proliferation and tumor growth and its role as an independent predictor of patient survival.  |  Cui, H., et al. 2016. Neurosci Lett. 631: 40-49. PMID: 27495011
5. NF-Y in cancer: Impact on cell transformation of a gene essential for proliferation.  |  Gurtner, A., et al. 2017. Biochim Biophys Acta Gene Regul Mech. 1860: 604-616. PMID: 27939755
6. An autoregulatory loop controls the expression of the transcription factor NF-Y.  |  Belluti, S., et al. 2018. Biochim Biophys Acta Gene Regul Mech. 1861: 509-518. PMID: 29505822
7. NF-YA Overexpression in Lung Cancer: LUSC.  |  Bezzecchi, E., et al. 2019. Genes (Basel). 10: PMID: 31744190
8. NF-Y Overexpression in Liver Hepatocellular Carcinoma (HCC).  |  Bezzecchi, E., et al. 2020. Int J Mol Sci. 21: PMID: 33271832
9. NF-Y subunits overexpression in gastric adenocarcinomas (STAD).  |  Gallo, A., et al. 2021. Sci Rep. 11: 23764. PMID: 34887475
10. NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.  |  Liu, Z., et al. 2023. Cell Rep. 42: 112963. PMID: 37561631

The preferred NF-YC antibody is NF-YC Antibody (G-12): sc-390985.