neuroglobin Double Nickase Plasmid (m): sc-425686-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- neuroglobin Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- neuroglobin Double Nickase Plasmid (m) and neuroglobin Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ngb. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
neuroglobin Double Nickase Plasmid (m) | sc-425686-NIC | 20 µg | $410.00 | |||
neuroglobin Double Nickase Plasmid (m2) | sc-425686-NIC-2 | 20 µg | $410.00 |
Mouse Ngb encodes neuroglobin, a heme-binding globin enriched in neurons that supports cellular oxygen handling and redox homeostasis under fluctuating metabolic demand. Neuroglobin participates in mitochondrial stress responses by modulating reactive oxygen and nitrogen species and is linked to preservation of respiratory chain function during hypoxia and reoxygenation. In neural tissues, Ngb expression correlates with pathways controlling apoptosis, oxidative phosphorylation, and nitric oxide signaling, making it relevant to studies of neuronal vulnerability and adaptive resilience. Altered neuroglobin regulation has been investigated in models of ischemic injury and neurodegenerative processes where oxidative stress and mitochondrial dysfunction contribute to pathology.
neuroglobin Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ngb locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ngb. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ngb function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ngb-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.