Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NDH II Double Nickase Plasmid (h): sc-402757-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NDH II Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NDH II Double Nickase Plasmid (h) and NDH II Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DHX9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NDH II Antibody (B-9): sc-137232
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NDH II Double Nickase Plasmid (h) | sc-402757-NIC | 20 µg | $410.00 | |||
NDH II Double Nickase Plasmid (h2) | sc-402757-NIC-2 | 20 µg | $410.00 |
DHX9 encodes NDH II, a multifunctional ATP-dependent DExH-box helicase that binds DNA and RNA to remodel nucleic acid structures during transcription, RNA processing, and genome maintenance. NDH II participates in R-loop surveillance and resolution, supports replication fork progression, and interfaces with DNA damage response pathways, including mechanisms coupled to homologous recombination and checkpoint signaling. Through its roles in coordinating transcription with RNA metabolism and preserving genome stability, DHX9 is frequently examined in studies of stress responses and dysregulated RNA–DNA hybrid homeostasis. Altered DHX9 activity has been linked to aberrant splicing programs and chromosomal instability phenotypes that are relevant to mechanistic research in cancer biology and neurodegeneration-associated genomic stress.
NDH II Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DHX9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DHX9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DHX9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DHX9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.