Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NCS-1 CRISPR/Cas9 KO Plasmid (h): sc-404303
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NCS-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NCS-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NCS-1 Antibody (G-4): sc-376206
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NCS-1 CRISPR/Cas9 KO Plasmid (h) | sc-404303 | 20 µg | $397.00 |
Overview
NCS1 encodes neuronal calcium sensor-1 (NCS-1), a conserved EF-hand Ca²⁺-binding protein that translates intracellular calcium transients into signaling outputs at membranes and synapses. NCS-1 regulates processes including vesicle trafficking and exocytosis, neurite outgrowth, and modulation of receptor and ion channel function through interactions with targets such as phosphatidylinositol 4-kinases and signaling complexes that shape phosphoinositide-dependent pathways. In human cells, NCS-1 contributes to calcium-dependent control of neuronal excitability and synaptic plasticity, linking stimulus-evoked Ca²⁺ flux to downstream adaptive responses. Altered NCS-1 expression or signaling has been investigated in the context of neuropsychiatric and neurodegenerative phenotypes, supporting its relevance for mechanistic studies of calcium signaling dysregulation.
NCS-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NCS1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NCS1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NCS1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NCS-1 protein expression.
This CRISPR knockout system enables efficient generation of NCS1-deficient cell models for investigation of NCS-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NCS1 exon(s) critical for NCS-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NCS1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NCS-1 CRISPR/Cas9 KO Plasmid (h) and NCS-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NCS1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NCS-1 HDR Plasmid (h) and NCS-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NCS1 homology arms to support homology-directed repair at defined NCS1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.