Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Nanog Double Nickase Plasmid (h): sc-418033-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nanog Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nanog Double Nickase Plasmid (h) and Nanog Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NANOG. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nanog Antibody (1E6C4): sc-293121
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nanog Double Nickase Plasmid (h) | sc-418033-NIC | 20 µg | $410.00 | |||
Nanog Double Nickase Plasmid (h2) | sc-418033-NIC-2 | 20 µg | $410.00 |
NANOG encodes Nanog, a homeobox transcription factor that is central to maintenance of human pluripotency and self-renewal by coordinating transcriptional networks with OCT4/POU5F1 and SOX2. Nanog influences cell fate decisions by regulating chromatin state and pluripotency-associated gene expression programs, interfacing with signaling inputs such as TGF-β/SMAD, WNT/β-catenin, and FGF/ERK pathways. Dysregulated NANOG expression is associated with aberrant differentiation, epigenetic instability, and stem-like transcriptional states observed in developmental disorders and diverse cancers. Consequently, NANOG is widely studied as a marker and functional regulator of pluripotent stem cells, reprogramming, and tumor cell plasticity.
Nanog Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NANOG locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NANOG. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NANOG function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NANOG-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.