N-type Ca CP α1B Double Nickase Plasmid (h): sc-402397-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- N-type Ca CP α1B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- N-type Ca CP α1B Double Nickase Plasmid (h) and N-type Ca CP α1B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CACNA1B. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
N-type Ca CP α1B Double Nickase Plasmid (h) | sc-402397-NIC | 20 µg | $410.00 | |||
N-type Ca CP α1B Double Nickase Plasmid (h2) | sc-402397-NIC-2 | 20 µg | $410.00 |
CACNA1B encodes the α1B pore-forming subunit of N-type voltage-gated calcium channels (CaV2.2), which mediate depolarization-evoked Ca2+ influx in excitable cells. This channel couples membrane electrical activity to presynaptic vesicle fusion and neurotransmitter release, integrating into Ca2+-dependent signaling networks that regulate synaptic plasticity, neuronal excitability, and activity-dependent gene expression. CACNA1B function is closely linked to modulation by G protein–coupled receptors and downstream pathways controlling synaptic transmission dynamics. Genetic variation or dysregulated CaV2.2 activity has been associated with altered nociceptive processing and neuropsychiatric phenotypes, supporting its relevance for mechanistic studies of neuronal circuit function.
N-type Ca CP α1B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CACNA1B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CACNA1B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CACNA1B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CACNA1B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.