Mucin 4/MUC4 Double Nickase Plasmid (h): sc-400329-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mucin 4/MUC4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Mucin 4/MUC4 Double Nickase Plasmid (h) and Mucin 4/MUC4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MUC4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Mucin 4/MUC4 Antibody (1G8): sc-33654
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mucin 4/MUC4 Double Nickase Plasmid (h) | sc-400329-NIC | 20 µg | $410.00 | |||
Mucin 4/MUC4 Double Nickase Plasmid (h2) | sc-400329-NIC-2 | 20 µg | $410.00 |
MUC4 encodes mucin 4, a high–molecular weight, membrane-associated glycoprotein that contributes to epithelial barrier function, lubrication, and formation of the glycocalyx through extensive O-glycosylation. As a cell-surface mucin, MUC4 participates in cell–cell and cell–matrix interactions and can modulate receptor signaling networks, including ERBB family-associated pathways, influencing proliferation, differentiation, and stress responses. Altered MUC4 expression and glycoform remodeling are frequently studied in the context of epithelial transformation, invasion-associated phenotypes, and immune evasion mechanisms at mucosal interfaces. These features make MUC4 a useful marker and functional node for investigating mucin biology, epithelial homeostasis, and signaling crosstalk in disease-relevant models.
Mucin 4/MUC4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MUC4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MUC4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MUC4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MUC4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.