MPDZ Double Nickase Plasmid (h): sc-406839-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MPDZ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MPDZ Double Nickase Plasmid (h) and MPDZ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MPDZ. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MPDZ Double Nickase Plasmid (h) | sc-406839-NIC | 20 µg | $410.00 | |||
MPDZ Double Nickase Plasmid (h2) | sc-406839-NIC-2 | 20 µg | $410.00 |
MPDZ encodes a multi-PDZ domain scaffold protein that organizes signaling and trafficking complexes at epithelial and neuronal cell junctions, contributing to tight junction assembly, apical–basal polarity, and regulation of membrane protein localization. By linking transmembrane partners to cytoskeletal and signaling effectors, MPDZ influences processes such as cell–cell adhesion, barrier function, and junctional remodeling. Altered MPDZ function has been associated with defects in neurodevelopment and epithelial integrity, including reported links to hydrocephalus and junction-related pathologies. As a hub-like adaptor, MPDZ is frequently studied to understand how PDZ-mediated interactions coordinate polarity networks and junctional signaling dynamics.
MPDZ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MPDZ locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MPDZ. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MPDZ function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MPDZ-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.