Mnk2 CRISPR/Cas9 KO Plasmid (m): sc-421658
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mnk2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Mnk2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Mnk2 Antibody (B-6): sc-271559
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mnk2 CRISPR/Cas9 KO Plasmid (m) | sc-421658 | 20 µg | $397.00 |
Overview
Mouse Mknk2 encodes MAP kinase–interacting serine/threonine-protein kinase 2 (Mnk2), a downstream effector of ERK and p38 MAPK signaling that modulates translation initiation by phosphorylating eIF4E and related components of the mRNA cap-dependent translation machinery. Through this node, Mnk2 integrates stress and mitogenic cues to shape selective mRNA translation, impacting cell growth, survival, and inflammatory responses. Altered MNK–eIF4E axis activity has been linked to dysregulated protein synthesis programs observed in cancer biology and immune-mediated pathologies, making Mknk2 a useful entry point for probing MAPK-driven translational control in mouse models. Functional interrogation of Mnk2 also supports studies of signaling cross-talk, immediate early gene responses, and context-specific translation under stress.
Mnk2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Mknk2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Mknk2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Mknk2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Mnk2 protein expression.
This CRISPR knockout system enables efficient generation of Mknk2-deficient cell models for investigation of Mnk2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Mknk2 exon(s) critical for Mnk2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Mknk2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Mnk2 CRISPR/Cas9 KO Plasmid (m) and Mnk2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Mknk2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Mnk2 HDR Plasmid (m) and Mnk2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Mknk2 homology arms to support homology-directed repair at defined Mknk2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.