Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MLL Double Nickase Plasmid (h): sc-401307-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MLL Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MLL Double Nickase Plasmid (h) and MLL Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KMT2A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MLL Antibody (D-3): sc-377274
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MLL Double Nickase Plasmid (h) | sc-401307-NIC | 20 µg | $410.00 | |||
MLL Double Nickase Plasmid (h2) | sc-401307-NIC-2 | 20 µg | $410.00 |
KMT2A encodes the histone-lysine N-methyltransferase MLL, a core regulator of chromatin state that catalyzes H3K4 methylation at promoters and enhancers to support transcriptional programs controlling hematopoietic development and cell-fate decisions. MLL functions within multiprotein complexes that coordinate transcriptional initiation and elongation and interfaces with pathways governing HOX gene expression and epigenetic memory. Disruption, rearrangement, or altered regulation of KMT2A perturbs gene-expression networks and is strongly implicated in leukemogenesis and broader epigenetic dysregulation, making it a key node for mechanistic studies of transcriptional control. Investigations commonly examine MLL-dependent chromatin remodeling, differentiation blockade, and interactions with cofactors that tune lineage-specific transcription.
MLL Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KMT2A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KMT2A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KMT2A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KMT2A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.