MKP-6 CRISPR/Cas9 KO Plasmid (h): sc-406250
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MKP-6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MKP-6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MKP-6 Antibody (4B5-E6): sc-517023
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MKP-6 CRISPR/Cas9 KO Plasmid (h) | sc-406250 | 20 µg | $397.00 |
Overview
Dual specificity protein phosphatase 14 (DUSP14), also known as MKP-6, is a cytoplasmic MAPK phosphatase that dephosphorylates threonine and tyrosine residues to attenuate MAPK signaling. It primarily modulates stress- and cytokine-responsive pathways, including JNK and p38 MAPK cascades, shaping downstream transcriptional programs that control proliferation, apoptosis, and inflammatory responses. Through its role as a negative feedback regulator of MAPK activity, DUSP14 helps tune signal amplitude and duration in immune and epithelial contexts. Dysregulated DUSP14 expression or activity has been associated with altered signaling homeostasis observed in cancer biology and immune-mediated disease mechanisms, supporting its use as a node for pathway-centric studies.
MKP-6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DUSP14 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DUSP14 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DUSP14 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MKP-6 protein expression.
This CRISPR knockout system enables efficient generation of DUSP14-deficient cell models for investigation of MKP-6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DUSP14 exon(s) critical for MKP-6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DUSP14 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MKP-6 CRISPR/Cas9 KO Plasmid (h) and MKP-6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DUSP14 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MKP-6 HDR Plasmid (h) and MKP-6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DUSP14 homology arms to support homology-directed repair at defined DUSP14 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.