Anticorpo MIF (D-2) raccomandato per il rilevamento di MIF di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo MIF (D-2): sc-271631

Anticorpo MIF (D-2) valutazione 4.4 di 5 di 11.
  • y_2021, m_9, d_20, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_it_IT, sid_271631, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 121ms
  • REVIEWS, PRODUCT
loading
5
7
4
3
3
0
2
0
1
1

Schede tecniche

    • Anti-MIF Antibody D-2 è un monoclonale di topo IgM κ MIF antibody, citato in 10 pubblicazioni, fornito in 200 µg/ml
    • specifico per un epitopo mappato tra gli amminoacidi 7-39 al N-terminus di MIF di origine human
    • raccomandato per il rilevamento di MIF di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA; anche reattivo con ulteriori specie, incluse porcine
    • disponibile coniugato ad agarosio for IP; HRP for WB, IHC(P) and ELISA; phycoerythrin, fluorescein, Alexa Fluor® 488 or Alexa Fluor® 647 per IF, IHC(P) e FCM
    • Contattaci per ricevere un FREE 10 µg sample di MIF (D-2): sc-271631.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MIF Antibody (D-2). This work is in progress.
loading

Is this antibody suitable for neutralization experiments in mice?

Richiesta da: Al2995
Thank you for your question. We have not tested the antibody for neutralization experiments and, therefore, we are uncertain if it will work in that application.
Risposta da: Santa Cruz TS
Data di pubblicazione: 2021-07-10

What application is the blocking peptide sc-271631 P appropriate for?

Richiesta da: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Risposta da: Technical Support
Data di pubblicazione: 2017-02-24
  • y_2021, m_9, d_20, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_it_IT, sid_271631, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 120ms
  • QUESTIONS, PRODUCT
Rated 4 di 5 di da Good antibody! Works well enough! I got strong bands at 1:500 dilution using 40ug of protein
Data di pubblicazione: 2018-11-08
Rated 5 di 5 di da Excellent For WB In MCF7 and T47D cells, diluted at 1:1000, using tricine SDS PAGE, a very specific band was abserved. Excellent antibody.
Data di pubblicazione: 2018-04-12
Rated 1 di 5 di da DId not detect mouse MIF (HT22 cells) Unfortunately, the antibody did not detect mouse MIF (HT22 & MEF cells) or rat MIF (primary rat cortex tissue) but human MIF (HEK293T cells).
Data di pubblicazione: 2018-01-08
Rated 5 di 5 di da WB detection We usually used this antibody for WB detection MIF protein,we got the specific stainingat dilution 1:250,it's shows good quality.
Data di pubblicazione: 2017-09-03
Rated 4 di 5 di da Work in IHC Good staining with low background using IHC in mouse tissue
Data di pubblicazione: 2017-08-28
Rated 5 di 5 di da Good for WB 1:500 diluted for wb of rat protein which extracted from h9c2,the band is strong ,but there exist another band below the main band.
Data di pubblicazione: 2017-08-08
Rated 5 di 5 di da Strong and reliable for IHC and WB Excellent choice if you need a dependable MIF antibody for immunohistochemistry or Western blot. Smart choice!
Data di pubblicazione: 2017-02-06
Rated 4 di 5 di da Decent band in ARPE-19 cell lysates Decent band in ARPE-19 cell lysates, correct molecular weight. Couple higher molecular weight non-specific bands.
Data di pubblicazione: 2016-10-05
  • y_2021, m_9, d_20, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_it_IT, sid_271631, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT