MAG CRISPR Activation Plasmid (h): sc-401604-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAG CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- MAG CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by MAG CRISPR Activation Plasmid (h) and MAG CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the MAG transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MAG Antibody (A-11): sc-166849
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAG CRISPR Activation Plasmid (h) | sc-401604-ACT | 20 µg | $397.00 |
Myelin associated glycoprotein (MAG) is a type I transmembrane immunoglobulin superfamily member enriched on oligodendrocytes and Schwann cells where it mediates axon–glia adhesion and supports myelin stability. Through recognition of sialylated gangliosides and related neuronal glycoconjugates, MAG influences neurite outgrowth, axonal caliber maintenance, and long-term myelinated fiber integrity. MAG participates in pathways that govern myelin–axon interactions and post-injury axonal growth inhibition, linking its activity to mechanisms of neural plasticity and repair. Altered MAG expression or immune reactivity to MAG has been associated with demyelinating and neurodegenerative contexts, making it a relevant target for studying white matter biology.
MAG CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous MAG expression without altering the underlying DNA sequence.
MAG CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the MAG locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the MAG transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous MAG expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native MAG locus and enabling the study of MAG-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of MAG pathway restoration in tumor cells with silenced or reduced MAG expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.