Lutropin β Double Nickase Plasmid (h): sc-403297-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Lutropin β Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Lutropin β Double Nickase Plasmid (h) and Lutropin β Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LHB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Lutropin β Antibody (C-6): sc-373941
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lutropin β Double Nickase Plasmid (h) | sc-403297-NIC | 20 µg | $410.00 | |||
Lutropin β Double Nickase Plasmid (h2) | sc-403297-NIC-2 | 20 µg | $410.00 |
LHB encodes the beta subunit of luteinizing hormone (LH), a pituitary glycoprotein hormone that heterodimerizes with the common alpha subunit (CGA) to form bioactive LH. Secreted LH binds the LHCGR receptor on gonadal target cells to activate cAMP/PKA signaling, driving steroidogenesis, ovulation, and reproductive tissue maturation within the hypothalamic–pituitary–gonadal axis. LHB expression is regulated by pulsatile gonadotropin-releasing hormone and is integrated with feedback from sex steroids and inhibins/activins to coordinate reproductive endocrine homeostasis. Dysregulated LH production or signaling is implicated in disorders of gonadal function and reproductive development, supporting its use in mechanistic studies of endocrine regulation and fertility-related pathways.
Lutropin β Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LHB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LHB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LHB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LHB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.