Loricrin Double Nickase Plasmid (h): sc-402452-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Loricrin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Loricrin Double Nickase Plasmid (h) and Loricrin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LOR. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Loricrin Double Nickase Plasmid (h) | sc-402452-NIC | 20 µg | $410.00 | |||
Loricrin Double Nickase Plasmid (h2) | sc-402452-NIC-2 | 20 µg | $410.00 |
Human LOR encodes loricrin, a highly abundant structural protein of the epidermal cornified envelope that becomes extensively crosslinked by transglutaminases during terminal keratinocyte differentiation. Loricrin contributes to stratum corneum barrier formation, mechanical resilience, and hydration control, integrating with cornification programs coordinated by calcium signaling, keratinization networks, and epidermal differentiation complex gene regulation. Altered LOR expression or envelope assembly is linked to impaired barrier function and inflammatory skin phenotypes, and pathogenic variants in loricrin have been associated with inherited keratinization disorders such as Vohwinkel syndrome and progressive symmetric erythrokeratoderma. As a barrier-associated gene, LOR is frequently studied in models of epidermal stress responses, wound repair, and differentiation-dependent transcriptional remodeling.
Loricrin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LOR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LOR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LOR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LOR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.