Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Lck CRISPR/Cas9 KO Plasmid (m): sc-421397
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Lck CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Lck genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Lck Antibody (3A5): sc-433
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lck CRISPR/Cas9 KO Plasmid (m) | sc-421397 | 20 µg | $397.00 |
Overview
Lck (lymphocyte-specific protein tyrosine kinase) is a Src family kinase that initiates T cell receptor (TCR) signaling by phosphorylating CD3 ITAM motifs and promoting recruitment and activation of ZAP70, leading to downstream LAT/SLP-76 signalosome assembly. In mouse immune cells, Lck regulates proximal signaling events that couple antigen recognition to calcium flux, MAPK activation, and transcriptional programs controlled by NFAT, NF-κB, and AP-1, shaping thymocyte development and peripheral T cell activation. Lck activity is integrated with co-receptor signaling (CD4/CD8) and is modulated by phosphorylation-dependent switching between active and inactive conformations, making it central to lymphocyte signaling thresholds. Dysregulated Lck-dependent pathways are frequently used as models for altered immune activation, lymphoproliferation, and aberrant tyrosine kinase signaling in hematopoietic contexts.
Lck CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Lck gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Lck together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Lck open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Lck protein expression.
This CRISPR knockout system enables efficient generation of Lck-deficient cell models for investigation of Lck signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Lck exon(s) critical for Lck function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Lck genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Lck CRISPR/Cas9 KO Plasmid (m) and Lck CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Lck locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Lck HDR Plasmid (m) and Lck HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Lck homology arms to support homology-directed repair at defined Lck target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.