Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Layilin Double Nickase Plasmid (h): sc-408326-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Layilin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Layilin Double Nickase Plasmid (h) and Layilin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LAYN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Layilin Antibody (C-7): sc-377389
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Layilin Double Nickase Plasmid (h) | sc-408326-NIC | 20 µg | $410.00 | |||
Layilin Double Nickase Plasmid (h2) | sc-408326-NIC-2 | 20 µg | $410.00 |
LAYN encodes layilin, a transmembrane C-type lectin–like receptor enriched at membrane ruffles and adhesion sites where it links extracellular cues to the cortical actin cytoskeleton. Layilin associates with hyaluronan-rich microenvironments and interacts with cytoskeletal and signaling regulators to influence cell shape remodeling, motility, and adhesion-dependent signaling pathways. As a component of dynamic cell–matrix interfaces, LAYN is studied in contexts where migratory programs and immune–stromal interactions are altered, including tumor microenvironment biology and inflammatory tissue remodeling. Dysregulated LAYN expression has been reported across multiple disease-associated transcriptomic datasets, supporting its use as a mechanistic node for interrogating adhesion and migration phenotypes.
Layilin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LAYN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LAYN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LAYN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LAYN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.