LASP-1 Double Nickase Plasmid (h): sc-404630-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LASP-1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LASP-1 Double Nickase Plasmid (h) and LASP-1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LASP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LASP-1 Antibody (G-7): sc-374059
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LASP-1 Double Nickase Plasmid (h) | sc-404630-NIC | 20 µg | $410.00 | |||
LASP-1 Double Nickase Plasmid (h2) | sc-404630-NIC-2 | 20 µg | $410.00 |
LASP1 encodes LIM and SH3 domain protein 1 (LASP-1), an actin-binding adaptor enriched at focal adhesions, lamellipodia, and membrane ruffles where it coordinates cytoskeletal remodeling and cell–matrix interactions. Through interactions with F-actin–associated complexes and signaling nodes linked to focal adhesion turnover, LASP-1 contributes to cell migration, adhesion dynamics, and mechanotransduction. LASP-1 is frequently studied in contexts of altered motility and invasion, where dysregulated expression or localization correlates with tumor progression and metastatic phenotypes in multiple cancer types. Its subcellular targeting and scaffold functions also make it relevant for probing how actin architecture couples to growth factor and integrin-linked pathways.
LASP-1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LASP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LASP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LASP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LASP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.