LAMP-2 Antibody (M3/84.6.34): sc-81729

LAMP-2 Antibody (M3/84.6.34) is a rat monoclonal IgG1 antibody targeting an epitope mapped to an immunoadsorbent-purified mouse macrophage glycoprotein fraction, with reactivity in mouse, rat, and human species. LAMP-2 monoclonal antibody (M3/84.6.34) is validated for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). LAMP-2, also known as CD107B, is a glycosylated type I transmembrane protein predominantly localized to the lysosomal membrane. LAMP-2′s cellular location is critical for maintaining lysosomal integrity, protecting the membrane from enzymatic degradation, and regulating lysosomal acidity, which is essential for the function of hydrolases within the organelle. LAMP-2 mediates the fusion of autophagosomes with lysosomes during autophagy, facilitating the degradation and recycling of cellular components. Synthesized in the endoplasmic reticulum and glycosylated in the Golgi apparatus, LAMP-2 reaches lysosomes through a distinct intracellular trafficking pathway. LAMP-2′s location and function are essential for cellular homeostasis, and LAMP-2 dysregulation is implicated in lysosomal storage disorders such as Danon disease and in pathological processes like tumor metastasis. Anti-LAMP-2 antibody (M3/84.6.34) serves as an invaluable tool for studying lysosomal dynamics, autophagy, and disease mechanisms.

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LAMP-2 Antibody (M3/84.6.34) References:

1. LAMP-2 positive vacuolar myopathy with dilated cardiomyopathy.  |  Sugimoto, S., et al. 2007. Intern Med. 46: 757-60. PMID: 17541230
2. Phagosome maturation in unicellular eukaryote Paramecium: the presence of RILP, Rab7 and LAMP-2 homologues.  |  Wyroba, E., et al. 2007. Eur J Histochem. 51: 163-72. PMID: 17921111
3. Impaired autolysosome formation correlates with Lamp-2 depletion: role of apoptosis, autophagy, and necrosis in pancreatitis.  |  Fortunato, F., et al. 2009. Gastroenterology. 137: 350-60, 360.e1-5. PMID: 19362087
4. Danon disease. A novel mutation in the LAMP-2 gene and ophthalmic abnormality.  |  He, J., et al. 2014. Herz. 39: 877-9. PMID: 23955649
5. Indomethacin suppresses LAMP-2 expression and induces lipophagy and lipoapoptosis in rat enterocytes via the ER stress pathway.  |  Narabayashi, K., et al. 2015. J Gastroenterol. 50: 541-54. PMID: 25212253
6. Expression of Beclin-1 and LAMP-2 in Rat Hippocampus under Conditions of Simulated Gravity Overload in the Caudocranial Vector.  |  Smirnov, AV., et al. 2018. Bull Exp Biol Med. 165: 105-109. PMID: 29797121
7. Cycling of two endogenous lysosomal membrane proteins, lamp-2 and acid phosphatase, between the cell surface and lysosomes in cultured rat hepatocytes.  |  Akasaki, K., et al. 1993. J Biochem. 114: 598-604. PMID: 8276775
8. Assignment of O-glycan attachment sites to the hinge-like regions of human lysosomal membrane glycoproteins lamp-1 and lamp-2.  |  Carlsson, SR., et al. 1993. Arch Biochem Biophys. 304: 65-73. PMID: 8323299
9. The lysosomal granule membrane protein, LAMP-2, is also present in platelet dense granule membranes.  |  Israels, SJ., et al. 1996. Thromb Haemost. 75: 623-9. PMID: 8743190
10. Evaluation of the lysosome-associated membrane protein LAMP-2 as a marker for lysosomal storage disorders.  |  Hua, CT., et al. 1998. Clin Chem. 44: 2094-102. PMID: 9761240

The preferred LAMP-2 antibody is LAMP-2 Antibody (H4B4): sc-18822.