Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Lamin B2 Double Nickase Plasmid (m): sc-421446-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Lamin B2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Lamin B2 Double Nickase Plasmid (m) and Lamin B2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Lmnb2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Lamin B2 Antibody (F-8): sc-377379
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lamin B2 Double Nickase Plasmid (m) | sc-421446-NIC | 20 µg | $410.00 | |||
Lamin B2 Double Nickase Plasmid (m2) | sc-421446-NIC-2 | 20 µg | $410.00 |
Mouse Lmnb2 encodes lamin B2, a core intermediate filament of the nuclear lamina that provides structural support to the nuclear envelope and helps organize peripheral chromatin. Lamin B2 participates in nuclear architecture, DNA replication timing, and mitotic reassembly of the nuclear envelope, linking lamina dynamics to cell-cycle progression and genome stability. Through interactions with lamina-associated domains, it contributes to transcriptional regulation and higher-order chromatin organization. Dysregulation of lamin B2 and related lamina components is relevant to laminopathies and broader mechanisms of nuclear fragility, altered gene expression programs, and stress responses studied in developmental and degenerative contexts.
Lamin B2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Lmnb2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Lmnb2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Lmnb2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Lmnb2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.