Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Lamin B1 Double Nickase Plasmid (h): sc-400032-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Lamin B1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Lamin B1 Double Nickase Plasmid (h) and Lamin B1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LMNB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Lamin B1 Antibody (A-11): sc-377000
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lamin B1 Double Nickase Plasmid (h) | sc-400032-NIC | 20 µg | $410.00 | |||
Lamin B1 Double Nickase Plasmid (h2) | sc-400032-NIC-2 | 20 µg | $410.00 |
LMNB1 encodes lamin B1, a core component of the nuclear lamina that polymerizes beneath the inner nuclear membrane to maintain nuclear shape, chromatin organization, and mechanotransduction. Lamin B1 participates in mitotic nuclear envelope breakdown and reassembly, regulates DNA replication and repair, and influences transcription through lamina-associated domains that position heterochromatin at the nuclear periphery. Altered lamin B1 dosage or organization has been linked to defects in myelination and neurodegenerative phenotypes, and more broadly to pathways governing genome stability, cell cycle progression, and nuclear architecture. As a scaffold for signaling and chromatin interactions, lamin B1 is frequently studied in the context of cellular senescence, differentiation, and stress responses.
Lamin B1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LMNB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LMNB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LMNB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LMNB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.