Lamellipodin Antibody (A-8): sc-377492

Lamellipodin Antibody (A-8) is a mouse monoclonal IgG1 antibody that detects Lamellipodin in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Lamellipodin, also known as ras-associated and pleckstrin homology domains-containing protein 1 (RAPH1), is a 1302 amino acid member of the MRL family and plays a crucial role in mediating localized membrane signals, particularly at the tips of filopodia and lamellipodia, where Lamellipodin colocalizes with ENAH/VASP. Lamellipodin′s ability to facilitate cellular signaling and motility is vital for processes such as cell migration and tissue development, making Lamellipodin an important factor in both normal physiology and disease states. Notably, Lamellipodin is expressed in various tissues, with highest levels found in brain, heart, ovary, and developing embryo, while Lamellipodin expression is significantly downregulated in breast and ovarian cancers, as well as in metastatic osteosarcomas compared to primary tumors. Additionally, Lamellipodin has nine identified isoforms, including RMO1, RMO1a, RMO1b, RMO1c, RMO1ab, RMO1ac, RMO1bc, RMO1abc, and RMO1-RAPH1, which may contribute to diverse functional roles in cellular processes. Anti-Lamellipodin antibody (A-8) is an essential tool for researchers investigating dynamics of cell movement and molecular mechanisms underlying cancer progression.

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Lamellipodin Antibody (A-8) References:

1. Lamellipodin Is Important for Cell-to-Cell Spread and Actin-Based Motility in Listeria monocytogenes.  |  Wang, J., et al. 2015. Infect Immun. 83: 3740-8. PMID: 26169271
2. Lamellipodin promotes actin assembly by clustering Ena/VASP proteins and tethering them to actin filaments.  |  Hansen, SD. and Mullins, RD. 2015. Elife. 4: PMID: 26295568
3. A RIAM/lamellipodin-talin-integrin complex forms the tip of sticky fingers that guide cell migration.  |  Lagarrigue, F., et al. 2015. Nat Commun. 6: 8492. PMID: 26419705
4. Lamellipodin promotes invasive 3D cancer cell migration via regulated interactions with Ena/VASP and SCAR/WAVE.  |  Carmona, G., et al. 2016. Oncogene. 35: 5155-69. PMID: 26996666
5. Brain specific Lamellipodin knockout results in hyperactivity and increased anxiety of mice.  |  Bodo, C., et al. 2017. Sci Rep. 7: 5365. PMID: 28710397
6. Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation.  |  Dimchev, G., et al. 2020. J Cell Sci. 133: PMID: 32094266
7. Initiation and disassembly of filopodia tip complexes containing VASP and lamellipodin.  |  Cheng, KW. and Mullins, RD. 2020. Mol Biol Cell. 31: 2021-2034. PMID: 32579429
8. Mechanosensitive expression of lamellipodin promotes intracellular stiffness, cyclin expression and cell proliferation.  |  Brazzo, JA., et al. 2021. J Cell Sci. 134: PMID: 34152388
9. PtdIns(3,4)P2, Lamellipodin, and VASP coordinate actin dynamics during phagocytosis in macrophages.  |  Montaño-Rendón, F., et al. 2022. J Cell Biol. 221: PMID: 36165850
10. GSK3 and lamellipodin balance lamellipodial protrusions and focal adhesion maturation in mouse neural crest migration.  |  Dobson, L., et al. 2023. Cell Rep. 42: 113030. PMID: 37632751

The preferred Lamellipodin antibody is Lamellipodin Antibody (H-5): sc-390050.