Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
KIR6.2 Double Nickase Plasmid (h): sc-401139-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KIR6.2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- KIR6.2 Double Nickase Plasmid (h) and KIR6.2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KCNJ11. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KIR6.2 Antibody (B-9): sc-390104
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KIR6.2 Double Nickase Plasmid (h) | sc-401139-NIC | 20 µg | $410.00 | |||
KIR6.2 Double Nickase Plasmid (h2) | sc-401139-NIC-2 | 20 µg | $410.00 |
KCNJ11 encodes the inwardly rectifying potassium channel subunit KIR6.2, a core component of ATP-sensitive K+ (KATP) channels that couple cellular metabolic state to membrane excitability. In pancreatic beta cells, KIR6.2 helps set resting potential and regulates glucose-stimulated insulin secretion by integrating ATP/ADP-dependent gating with changes in cytosolic Ca2+ entry. In excitable tissues such as heart and skeletal muscle, KATP channels modulate action potential duration and protect against metabolic stress through electrophysiological adaptation. Genetic variation or altered regulation of KCNJ11 has been associated with disorders of insulin secretion and glucose homeostasis, making it a widely used target in metabolic signaling and ion channel physiology research.
KIR6.2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KCNJ11 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KCNJ11. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KCNJ11 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KCNJ11-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.