KIF4 Antibody (E-8): sc-365144
- KIF4 Antibody (E-8) is a mouse monoclonal IgG1 κ KIF4 antibody, cited in 10 publications, provided at 200 µg/ml
- raised against amino acids 361-660 mapping within an internal region of KIF4A of human origin
- KIF4 Antibody (E-8) is recommended for detection of KIF4A and KIF4B of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-KIF4 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for KIF4 Antibody (E-8) for WB applications. These reagents are now offered in bundles with KIF4 Antibody (E-8) (see ordering information below).
KIF4 Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects KIF4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). KIF4 Antibody (E-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. KIF4, also known as kinesin family member 4A, is a vital motor protein that plays a significant role in the transport of cellular components, particularly during cell division. KIF4 is crucial for proper positioning of chromosomes and stabilization of the bipolar spindle during mitosis, which is essential for accurate cell division and prevention of aneuploidy. KIF4 is predominantly expressed in hematopoietic tissues, including spleen, thymus, bone marrow, and fetal liver, highlighting KIF4′s importance in cellular processes within these critical areas. Additionally, KIF4 interacts with other proteins such as PRC1, facilitating PRC1′s translocation to interlocking spindle microtubules during metaphase to anaphase transition of cytokinesis. KIF4′s ability to mediate these interactions underscores KIF4′s essential role in maintaining cellular integrity and function, making anti-KIF4 antibody (E-8) a valuable tool for researchers studying cell division and intracellular transport mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
KIF4 Antibody (E-8) References:
- Identification of the human homologue of mouse KIF4, a kinesin superfamily motor protein. | Oh, S., et al. 2000. Biochim Biophys Acta. 1493: 219-24. PMID: 10978527
- Human kinesin superfamily member 4 is dominantly localized in the nuclear matrix and is associated with chromosomes during mitosis. | Lee, YM., et al. 2001. Biochem J. 360: 549-56. PMID: 11736643
- Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation. | Kurasawa, Y., et al. 2004. EMBO J. 23: 3237-48. PMID: 15297875
- Cell cycle-dependent translocation of PRC1 on the spindle by Kif4 is essential for midzone formation and cytokinesis. | Zhu, C. and Jiang, W. 2005. Proc Natl Acad Sci U S A. 102: 343-8. PMID: 15625105
- KIF14 and citron kinase act together to promote efficient cytokinesis. | Gruneberg, U., et al. 2006. J Cell Biol. 172: 363-72. PMID: 16431929
- The kinesin superfamily motor protein KIF4 is associated with immune cell activation in idiopathic inflammatory myopathies. | Bernasconi, P., et al. 2008. J Neuropathol Exp Neurol. 67: 624-32. PMID: 18520780
- A novel role of the chromokinesin Kif4A in DNA damage response. | Wu, G., et al. 2008. Cell Cycle. 7: 2013-20. PMID: 18604178
- Kinesin KIF4 regulates intracellular trafficking and stability of the human immunodeficiency virus type 1 Gag polyprotein. | Martinez, NW., et al. 2008. J Virol. 82: 9937-50. PMID: 18684836
- KIF4 mediates anterograde translocation and positioning of ribosomal constituents to axons. | Bisbal, M., et al. 2009. J Biol Chem. 284: 9489-97. PMID: 19158085
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KIF4 Antibody (E-8) | sc-365144 | 200 µg/ml | $322.00 | |||
KIF4 Antibody (E-8): m-IgG Fc BP-HRP Bundle | sc-529314 | 200 µg Ab; 10 µg BP | $361.00 | |||
KIF4 Antibody (E-8): m-IgGκ BP-HRP Bundle | sc-522144 | 200 µg Ab, 40 µg BP | $361.00 | |||
KIF4 Antibody (E-8): m-IgG1 BP-HRP Bundle | sc-543449 | 200 µg Ab; 20 µg BP | $361.00 | |||
KIF4 Antibody (E-8) AC | sc-365144 AC | 500 µg/ml, 25% agarose | $424.00 | |||
KIF4 Antibody (E-8) HRP | sc-365144 HRP | 200 µg/ml | $322.00 | |||
KIF4 Antibody (E-8) FITC | sc-365144 FITC | 200 µg/ml | $336.00 | |||
KIF4 Antibody (E-8) PE | sc-365144 PE | 200 µg/ml | $349.00 | |||
KIF4 Antibody (E-8) Alexa Fluor® 488 | sc-365144 AF488 | 200 µg/ml | $364.00 | |||
KIF4 Antibody (E-8) Alexa Fluor® 546 | sc-365144 AF546 | 200 µg/ml | $364.00 | |||
KIF4 Antibody (E-8) Alexa Fluor® 594 | sc-365144 AF594 | 200 µg/ml | $364.00 | |||
KIF4 Antibody (E-8) Alexa Fluor® 647 | sc-365144 AF647 | 200 µg/ml | $364.00 | |||
KIF4 Antibody (E-8) Alexa Fluor® 680 | sc-365144 AF680 | 200 µg/ml | $364.00 | |||
KIF4 Antibody (E-8) Alexa Fluor® 790 | sc-365144 AF790 | 200 µg/ml | $364.00 |