KCTD1 CRISPR/Cas9 KO Plasmid (h): sc-406758
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KCTD1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KCTD1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KCTD1 CRISPR/Cas9 KO Plasmid (h) | sc-406758 | 20 µg | $397.00 |
Overview
KCTD1 (potassium channel tetramerization domain containing 1) encodes a BTB/POZ domain–containing protein implicated in protein–protein interaction networks that influence transcriptional control and cellular differentiation programs. KCTD family members commonly function as adaptor proteins within Cullin3 (CUL3)-based E3 ubiquitin ligase complexes, linking specific substrates to ubiquitin-mediated turnover and thereby shaping signaling outputs. Through these regulatory roles, KCTD1 has been associated with developmental processes and maintenance of cell identity, with genetic disruption linked to congenital disorders affecting ectodermal tissues. In biomedical research, KCTD1 is studied in the context of pathway modulation, proteostasis, and gene regulatory circuitry relevant to disease phenotypes.
KCTD1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KCTD1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KCTD1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KCTD1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KCTD1 protein expression.
This CRISPR knockout system enables efficient generation of KCTD1-deficient cell models for investigation of KCTD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KCTD1 exon(s) critical for KCTD1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KCTD1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KCTD1 CRISPR/Cas9 KO Plasmid (h) and KCTD1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KCTD1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KCTD1 HDR Plasmid (h) and KCTD1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KCTD1 homology arms to support homology-directed repair at defined KCTD1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.