Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
JAK2 Double Nickase Plasmid (m): sc-421200-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- JAK2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- JAK2 Double Nickase Plasmid (m) and JAK2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Jak2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: JAK2 Antibody (C-10): sc-390539
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
JAK2 Double Nickase Plasmid (m) | sc-421200-NIC | 20 µg | $410.00 | |||
JAK2 Double Nickase Plasmid (m2) | sc-421200-NIC-2 | 20 µg | $410.00 |
Mouse Jak2 encodes the non-receptor tyrosine kinase JAK2, a central mediator of cytokine receptor signaling that couples ligand engagement to phosphorylation of STAT transcription factors. JAK2 activity integrates with JAK–STAT, MAPK/ERK, and PI3K–AKT pathways to regulate hematopoiesis, immune cell differentiation, inflammation, and metabolic homeostasis. In vivo and cellular studies link Jak2 perturbation to altered erythropoietin and thrombopoietin responses, stress signaling, and dysregulated growth control, making it relevant to models of myeloproliferation and immune dysfunction. As a signaling hub, JAK2 is frequently used to dissect receptor proximal kinase cascades and transcriptional programs downstream of cytokines.
JAK2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Jak2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Jak2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Jak2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Jak2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.