Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
JAB1 Double Nickase Plasmid (h): sc-401302-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- JAB1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- JAB1 Double Nickase Plasmid (h) and JAB1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting COPS5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: JAB1 Antibody (B-7): sc-13157
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
JAB1 Double Nickase Plasmid (h) | sc-401302-NIC | 20 µg | $410.00 | |||
JAB1 Double Nickase Plasmid (h2) | sc-401302-NIC-2 | 20 µg | $410.00 |
COPS5 encodes JAB1 (CSN5), the catalytic subunit of the COP9 signalosome that regulates cullin-RING ubiquitin ligases through deneddylation, thereby shaping ubiquitin-dependent protein turnover. By modulating stability and activity of key cell-cycle and stress-response regulators, JAB1 influences DNA damage responses, signal transduction, and transcriptional programs linked to proliferation and differentiation. JAB1 also interfaces with pathways such as AP-1 signaling and proteostasis networks that coordinate cellular adaptation to environmental cues. Dysregulated COPS5/JAB1 activity has been associated with altered growth control and oncogenic phenotypes, making it a frequent target in mechanistic studies of tumor-relevant signaling and protein degradation.
JAB1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the COPS5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within COPS5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt COPS5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of COPS5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.