Islet-1 CRISPR/Cas9 KO Plasmid (m): sc-421157
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Islet-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Islet-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Islet-1 Antibody (B-1): sc-390793
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Islet-1 CRISPR/Cas9 KO Plasmid (m) | sc-421157 | 20 µg | $397.00 |
Overview
Mouse Isl1 encodes the LIM-homeodomain transcription factor Islet-1 (ISL1), a key regulator of lineage specification and differentiation in the developing nervous system, pancreas, and cardiovascular tissues. ISL1 coordinates transcriptional programs that control progenitor maintenance, cell fate decisions, and maturation, acting through context-dependent interactions with LIM-binding cofactors and homeobox networks. In pancreatic islet biology, Isl1 supports endocrine gene expression and functional maturation, while in motor neuron and cardiac progenitors it contributes to patterning and morphogenesis. Dysregulated ISL1 activity is therefore relevant to mechanistic studies of diabetes-related islet dysfunction, congenital heart development, and neurodevelopmental pathways in mouse models.
Islet-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Isl1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Isl1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Isl1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Islet-1 protein expression.
This CRISPR knockout system enables efficient generation of Isl1-deficient cell models for investigation of Islet-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Isl1 exon(s) critical for Islet-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Isl1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Islet-1 CRISPR/Cas9 KO Plasmid (m) and Islet-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Isl1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Islet-1 HDR Plasmid (m) and Islet-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Isl1 homology arms to support homology-directed repair at defined Isl1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.