Integrin β8/ITGB8 Double Nickase Plasmid (h): sc-401379-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin β8/ITGB8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin β8/ITGB8 Double Nickase Plasmid (h) and Integrin β8/ITGB8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITGB8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin β8/ITGB8 Antibody (E-6): sc-514150
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin β8/ITGB8 Double Nickase Plasmid (h) | sc-401379-NIC | 20 µg | $410.00 | |||
Integrin β8/ITGB8 Double Nickase Plasmid (h2) | sc-401379-NIC-2 | 20 µg | $410.00 |
ITGB8 encodes integrin β8, a single-pass transmembrane adhesion receptor subunit that pairs with integrin αV to form αVβ8, linking extracellular matrix ligands to intracellular cytoskeletal and signaling networks. αVβ8 is a key regulator of latent TGF-β activation at the cell surface, shaping SMAD-dependent transcriptional programs that influence epithelial–mesenchymal interactions, immune modulation, and tissue remodeling. Through integrin-mediated outside-in signaling and focal adhesion dynamics, ITGB8 contributes to cell adhesion, migration, and barrier organization. Dysregulated ITGB8 expression or αVβ8 activity has been associated with altered TGF-β signaling states observed across contexts such as tumor microenvironment remodeling, fibrotic processes, and neurovascular development.
Integrin β8/ITGB8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ITGB8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ITGB8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ITGB8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ITGB8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.