Integrin β6/ITGB6 Double Nickase Plasmid (m): sc-421178-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin β6/ITGB6 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin β6/ITGB6 Double Nickase Plasmid (m) and Integrin β6/ITGB6 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Itgb6. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin β6/ITGB6 Double Nickase Plasmid (m) | sc-421178-NIC | 20 µg | $410.00 | |||
Integrin β6/ITGB6 Double Nickase Plasmid (m2) | sc-421178-NIC-2 | 20 µg | $410.00 |
Mouse Itgb6 encodes integrin β6, a β subunit that pairs with αv to form the epithelial-restricted αvβ6 integrin, a key mediator of cell–extracellular matrix adhesion and mechanotransduction. αvβ6 binds RGD-containing ligands such as fibronectin and latency-associated peptides, promoting activation of latent TGF-β and coupling to focal adhesion kinase/Src signaling that regulates epithelial remodeling, migration, and differentiation. Through integrin-dependent signaling and TGF-β pathway crosstalk, ITGB6 influences wound repair programs, fibrotic remodeling, and tumor–stroma interactions in multiple tissues. Dysregulated ITGB6 expression or function is therefore commonly examined in models of inflammation-driven tissue remodeling, fibrosis, and epithelial cancer progression.
Integrin β6/ITGB6 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Itgb6 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Itgb6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Itgb6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Itgb6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.