Integrin β6/ITGB6 Double Nickase Plasmid (h): sc-400999-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin β6/ITGB6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin β6/ITGB6 Double Nickase Plasmid (h) and Integrin β6/ITGB6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITGB6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin β6/ITGB6 Antibody (C5): sc-517598
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin β6/ITGB6 Double Nickase Plasmid (h) | sc-400999-NIC | 20 µg | $410.00 | |||
Integrin β6/ITGB6 Double Nickase Plasmid (h2) | sc-400999-NIC-2 | 20 µg | $410.00 |
ITGB6 encodes the integrin β6 subunit that pairs with integrin αv to form the epithelial-restricted αvβ6 heterodimer, a key mediator of cell–ECM adhesion and mechanotransduction. αvβ6 binds RGD-containing ligands such as fibronectin and vitronectin and can activate latent TGF-β via interaction with the LAP complex, linking ITGB6 to TGF-β/SMAD signaling, epithelial remodeling, and immune–stromal crosstalk. Through focal adhesion assembly and downstream pathways including FAK/SRC, MAPK, and PI3K signaling, ITGB6 influences migration, invasion, and wound repair programs. Dysregulated ITGB6 expression has been associated with fibrosis, chronic inflammation, and multiple epithelial cancers, where it is often studied as a driver of invasive phenotypes and microenvironmental signaling.
Integrin β6/ITGB6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ITGB6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ITGB6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ITGB6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ITGB6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.