Integrin α1/ITGA1/CD49a Double Nickase Plasmid (h): sc-401275-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin α1/ITGA1/CD49a Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin α1/ITGA1/CD49a Double Nickase Plasmid (h) and Integrin α1/ITGA1/CD49a Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITGA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin α1/ITGA1/CD49a Antibody (A-9): sc-271034
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin α1/ITGA1/CD49a Double Nickase Plasmid (h) | sc-401275-NIC | 20 µg | $410.00 | |||
Integrin α1/ITGA1/CD49a Double Nickase Plasmid (h2) | sc-401275-NIC-2 | 20 µg | $410.00 |
ITGA1 encodes integrin α1 (CD49a), which heterodimerizes with integrin β1 to form the α1β1 collagen/laminin receptor that mediates cell–extracellular matrix adhesion and bidirectional signaling. Through coupling to focal adhesion complexes and pathways including FAK/Src, PI3K–AKT, and MAPK, ITGA1 influences cytoskeletal remodeling, migration, survival, and mechanotransduction. CD49a is expressed in multiple tissue compartments and immune subsets, supporting processes such as tissue residency, adhesion to basement membranes, and matrix-dependent differentiation. Dysregulated ITGA1 signaling and altered cell–matrix interactions have been linked to fibrosis, inflammation, and tumor cell invasion and metastasis, making it a useful node for studying adhesion-driven phenotypes.
Integrin α1/ITGA1/CD49a Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ITGA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ITGA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ITGA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ITGA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.