Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ING2 CRISPR/Cas9 KO Plasmid (h): sc-404193
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ING2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ING2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ING2 Antibody (B-5): sc-271544
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ING2 CRISPR/Cas9 KO Plasmid (h) | sc-404193 | 20 µg | $397.00 |
Overview
ING2 (inhibitor of growth family member 2) is a nuclear tumor suppressor–associated protein that functions as a chromatin reader through its C-terminal PHD finger, recognizing H3K4me3 and coordinating transcriptional programs linked to DNA damage signaling and cell-cycle control. It participates in epigenetic regulation by engaging histone acetyltransferase/deacetylase complexes and modulating p53-dependent stress responses, thereby influencing apoptosis, senescence, and genome stability. ING2 has been connected to pathways controlling chromatin accessibility and repair factor recruitment following genotoxic stress. Altered ING2 expression or function has been reported in multiple cancer contexts and is studied for its contributions to oncogenic transformation, metastatic potential, and therapy resistance mechanisms at the molecular level.
ING2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ING2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ING2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ING2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ING2 protein expression.
This CRISPR knockout system enables efficient generation of ING2-deficient cell models for investigation of ING2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ING2 exon(s) critical for ING2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ING2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ING2 CRISPR/Cas9 KO Plasmid (h) and ING2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ING2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ING2 HDR Plasmid (h) and ING2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ING2 homology arms to support homology-directed repair at defined ING2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.