Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IL-23 CRISPR/Cas9 KO Plasmid (m): sc-429901
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-23 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IL-23 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-23 Antibody (C-3): sc-271279
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-23 CRISPR/Cas9 KO Plasmid (m) | sc-429901 | 20 µg | $397.00 |
Overview
Il23a encodes the p19 subunit of interleukin-23 (IL-23), a heterodimeric cytokine that pairs with IL-12p40 to form IL-23 and signals through the IL-23 receptor complex to activate JAK/STAT pathways, particularly STAT3. In myeloid cells such as dendritic cells and macrophages, IL-23 integrates pattern-recognition receptor inputs to reinforce inflammatory programs and promote type 17 immunity. IL-23 supports expansion and maintenance of Th17 cells and other IL-17–producing populations, linking innate sensing to IL-17A/F and IL-22 effector responses. Dysregulated Il23a/IL-23 activity is widely used as a mechanistic axis in mouse models of autoimmune and inflammatory pathology, including intestinal inflammation, psoriasis-like dermatitis, and CNS autoimmunity.
IL-23 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Il23a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Il23a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Il23a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IL-23 protein expression.
This CRISPR knockout system enables efficient generation of Il23a-deficient cell models for investigation of IL-23 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Il23a exon(s) critical for IL-23 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Il23a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IL-23 CRISPR/Cas9 KO Plasmid (m) and IL-23 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Il23a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IL-23 HDR Plasmid (m) and IL-23 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Il23a homology arms to support homology-directed repair at defined Il23a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.