Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IL-1RAcP CRISPR/Cas9 KO Plasmid (m): sc-421101
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-1RAcP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IL-1RAcP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-1RAcP Antibody (D-5): sc-376872
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-1RAcP CRISPR/Cas9 KO Plasmid (m) | sc-421101 | 20 µg | $397.00 |
Overview
Il1rap encodes IL-1RAcP, an essential co-receptor that complexes with IL-1 receptor family members to propagate signals initiated by IL-1α/IL-1β and related ligands. Upon receptor engagement, IL-1RAcP supports assembly of MyD88-dependent signaling complexes that activate IRAK–TRAF6 cascades, leading to NF-κB and MAPK pathway activation and downstream transcriptional programs controlling inflammation, innate immune responses, and stromal–immune communication. In mouse models, altered IL-1R/IL-1RAcP signaling is frequently used to study mechanisms driving chronic inflammatory states, tissue injury responses, and cytokine-amplified remodeling in diverse organ systems. Because IL-1RAcP integrates multiple IL-1 family inputs, it is relevant for dissecting pathway crosstalk that shapes leukocyte recruitment, barrier function, and inflammatory gene expression.
IL-1RAcP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Il1rap gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Il1rap together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Il1rap open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IL-1RAcP protein expression.
This CRISPR knockout system enables efficient generation of Il1rap-deficient cell models for investigation of IL-1RAcP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Il1rap exon(s) critical for IL-1RAcP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Il1rap genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IL-1RAcP CRISPR/Cas9 KO Plasmid (m) and IL-1RAcP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Il1rap locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IL-1RAcP HDR Plasmid (m) and IL-1RAcP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Il1rap homology arms to support homology-directed repair at defined Il1rap target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.