IFN-γ/Interferon gamma Antibody (E8H1): sc-101424

IFN-γ Antibody (E8H1) is a mouse monoclonal IgG1 antibody that detects Interferon gamma (IFN-γ) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). IFN-γ plays a crucial role in the immune response, acting as a potent antiviral and antiparasitic agent produced by activated CD4+/CD8+ lymphocytes and natural killer cells. Anti-IFN-γ antibody (E8H1) recognizes a protein that modulates T cell growth and differentiation while inhibiting B cell proliferation, thereby orchestrating a balanced immune response. IFN-γ′s active form is a homodimer, with each subunit comprising six helices, and this dimeric structure is stabilized by non-covalent interactions at the interface of the helices, which is essential for biological activity. The IFN-γ precursor consists of 166 amino acids, including a 23 amino acid secretory sequence, and functionality is further influenced by post-translational modifications such as glycosylation, which can lead to multiple isoforms under non-denaturing conditions, including dimers and tetramers. IFN-γ monoclonal antibody (E8H1) serves as an invaluable tool for studying immune responses in human samples, given IFN-γ′s versatility in immune regulation and significance in therapeutic applications and research.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

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IFN-γ/Interferon gamma Antibody (E8H1) References:

1. Interferon gamma (IFN-gamma) may reverse oral submucous fibrosis.  |  Haque, MF., et al. 2001. J Oral Pathol Med. 30: 12-21. PMID: 11140895
2. IFN-gamma affects homing of diabetogenic T cells.  |  Savinov, AY., et al. 2001. J Immunol. 167: 6637-43. PMID: 11714835
3. Immune control of tuberculosis by IFN-gamma-inducible LRG-47.  |  MacMicking, JD., et al. 2003. Science. 302: 654-9. PMID: 14576437
4. Prediction of IFN-gamma regulated gene transcription.  |  Liu, H., et al. 2004. In Silico Biol. 4: 489-505. PMID: 15506998
5. Influence of IFN- alpha and IFN- gamma on lymphangiogenesis.  |  Shao, X. and Liu, C. 2006. J Interferon Cytokine Res. 26: 568-74. PMID: 16881867
6. ISG15 regulates IFN-γ immunity in human mycobacterial disease.  |  Fan, JB. and Zhang, DE. 2013. Cell Res. 23: 173-5. PMID: 22964713
7. IFN-γ induces aberrant CD49b⁺ NK cell recruitment through regulating CX3CL1: a novel mechanism by which IFN-γ provokes pregnancy failure.  |  Li, ZY., et al. 2014. Cell Death Dis. 5: e1512. PMID: 25375377
8. IFN-gamma receptors on human tumor cells: relationship between receptor ligand interactions and induction of IFN-gamma response.  |  Berkovic, D., et al. 1986. Immunobiology. 172: 243-9. PMID: 2948902
9. Production of bioactive human IFN-γ protein by agroinfiltration in tobacco.  |  Heidari-Japelaghi, R., et al. 2020. Protein Expr Purif. 173: 105616. PMID: 32179088
10. Splenic T lymphocytes induce the formation of immunosuppressive neutrophils through IFN-γ in sepsis.  |  Huang, J., et al. 2022. Inflamm Res. 71: 81-91. PMID: 34841450

The preferred IFN-γ/Interferon gamma antibody is IFN-γ/Interferon gamma Antibody (G-23): sc-8423.