Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HMG-I/HMG-Y Double Nickase Plasmid (h): sc-402396-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HMG-I/HMG-Y Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HMG-I/HMG-Y Double Nickase Plasmid (h) and HMG-I/HMG-Y Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HMGA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HMG-I/HMG-Y Antibody (D-12): sc-393213
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HMG-I/HMG-Y Double Nickase Plasmid (h) | sc-402396-NIC | 20 µg | $410.00 | |||
HMG-I/HMG-Y Double Nickase Plasmid (h2) | sc-402396-NIC-2 | 20 µg | $410.00 |
HMGA1 encodes the chromatin-associated architectural proteins HMG-I and HMG-Y, which bind AT-rich DNA sequences to modulate nucleosome organization and higher-order chromatin structure. By facilitating enhanceosome assembly and altering DNA topology, HMGA1 influences transcriptional programs governing cell-cycle progression, differentiation, and stress-responsive signaling, including pathways linked to inflammatory gene regulation. Dysregulated HMGA1 expression and chromatin occupancy have been associated with altered proliferation and genome stability in multiple disease contexts, making it a key node in studies of oncogenic transcriptional rewiring. In human cells, HMGA1 is also implicated in regulation of replication timing and DNA damage responses through its effects on chromatin accessibility.
HMG-I/HMG-Y Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HMGA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HMGA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HMGA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HMGA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.