HLF Double Nickase Plasmid (h): sc-404752-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HLF Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HLF Double Nickase Plasmid (h) and HLF Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HLF. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HLF Antibody (4D8): sc-134359
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HLF Double Nickase Plasmid (h) | sc-404752-NIC | 20 µg | $410.00 | |||
HLF Double Nickase Plasmid (h2) | sc-404752-NIC-2 | 20 µg | $410.00 |
Human HLF (hepatic leukemia factor) encodes a PAR bZIP transcription factor that binds specific promoter elements to coordinate circadian-regulated gene expression and tissue-specific transcriptional programs. HLF participates in transcriptional networks controlling differentiation, metabolic homeostasis, and cellular stress responses through regulation of downstream target genes in liver and hematopoietic lineages. Dysregulated HLF activity has been linked to hematologic malignancy biology, including fusion-driven transcriptional reprogramming, and has also been studied in contexts of circadian disruption and altered metabolic phenotypes. As a nuclear DNA-binding protein, HLF is frequently investigated for its role in gene regulatory circuitry and lineage maintenance.
HLF Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HLF locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HLF. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HLF function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HLF-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.