Histamine H3 Receptor CRISPR Activation Plasmid (h): sc-402473-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Histamine H3 Receptor CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- Histamine H3 Receptor CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by Histamine H3 Receptor CRISPR Activation Plasmid (h) and Histamine H3 Receptor CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the HRH3 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Histamine H3 Receptor Antibody (D-5): sc-390140
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Histamine H3 Receptor CRISPR Activation Plasmid (h) | sc-402473-ACT | 20 µg | $397.00 | |||
Histamine H3 Receptor CRISPR Activation Plasmid (h2) | sc-402473-ACT-2 | 20 µg | $397.00 |
HRH3 encodes the histamine H3 receptor, a Gi/o-coupled GPCR predominantly expressed in the nervous system where it functions as an autoreceptor and heteroreceptor to modulate neurotransmitter release. Upon histamine binding, HRH3 signaling reduces intracellular cAMP, influences ion channel activity, and shapes synaptic transmission across pathways regulating arousal, cognition, and appetite. Through cross-talk with other neuromodulatory systems, HRH3 contributes to the control of dopaminergic, cholinergic, and serotonergic tone in neuronal circuits. Altered HRH3 expression or signaling has been investigated in the context of neuropsychiatric and neurodegenerative disease biology, sleep–wake regulation, and metabolic phenotypes.
Histamine H3 Receptor CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous HRH3 expression without altering the underlying DNA sequence.
Histamine H3 Receptor CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the HRH3 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the HRH3 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous Histamine H3 Receptor expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native HRH3 locus and enabling the study of Histamine H3 Receptor-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of Histamine H3 Receptor pathway restoration in tumor cells with silenced or reduced HRH3 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.