Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
hippocalcin CRISPR Activation Plasmid (m): sc-420938-ACT
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- hippocalcin CRISPR Activation Plasmid (m) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- hippocalcin CRISPR Activation Plasmid (m) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by hippocalcin CRISPR Activation Plasmid (m) and hippocalcin CRISPR Activation Plasmid (m2) target distinct regulatory regions upstream of the Hpca transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: hippocalcin Antibody (G-8): sc-393125
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hippocalcin CRISPR Activation Plasmid (m) | sc-420938-ACT | 20 µg | $397.00 | |||
hippocalcin CRISPR Activation Plasmid (m2) | sc-420938-ACT-2 | 20 µg | $397.00 |
Hpca encodes hippocalcin, a neuron-enriched EF-hand calcium-binding protein that functions as a calcium sensor linking activity-dependent Ca2+ transients to downstream signaling. Hippocalcin participates in synaptic plasticity mechanisms by modulating Ca2+-regulated protein interactions, membrane association via myristoyl switching, and endocytosis-related processes that shape neurotransmission. In mouse brain circuits, Hpca expression is associated with regulation of neuronal excitability and long-term synaptic changes, connecting it to pathways that influence learning and memory. Altered calcium homeostasis and synaptic signaling implicate hippocalcin-related networks in neurodegeneration and neuropsychiatric disease-relevant phenotypes used for mechanistic research models.
hippocalcin CRISPR Activation Plasmid (m) provides a targeted, non-destructive approach to upregulating endogenous Hpca expression without altering the underlying DNA sequence.
hippocalcin CRISPR Activation Plasmid (m) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the Hpca locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the Hpca transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous hippocalcin expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native Hpca locus and enabling the study of hippocalcin-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of hippocalcin pathway restoration in tumor cells with silenced or reduced Hpca expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.