Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Hic-5 Double Nickase Plasmid (h): sc-402786-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hic-5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Hic-5 Double Nickase Plasmid (h) and Hic-5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TGFB1I1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Hic-5 Antibody (C-6): sc-271353
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hic-5 Double Nickase Plasmid (h) | sc-402786-NIC | 20 µg | $410.00 | |||
Hic-5 Double Nickase Plasmid (h2) | sc-402786-NIC-2 | 20 µg | $410.00 |
TGFB1I1 encodes Hic-5 (also known as ARA55), a LIM domain-containing focal adhesion adaptor that integrates extracellular matrix cues with cytoskeletal remodeling and transcriptional responses. Hic-5 localizes to focal adhesions and the nucleus, where it scaffolds signaling complexes linked to integrin/FAK–SRC signaling, Rho family GTPase regulation, and mechanotransduction pathways that coordinate adhesion, migration, and stress fiber dynamics. It is inducible by TGF-β and participates in TGF-β–dependent remodeling programs, including context-specific effects on epithelial–mesenchymal transition and fibroblast activation. Dysregulated TGFB1I1/Hic-5 activity has been associated with cancer cell invasion and metastasis biology, fibrotic signaling networks, and inflammatory microenvironment remodeling, making it a useful node for studying adhesion-dependent transcriptional control.
Hic-5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TGFB1I1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TGFB1I1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TGFB1I1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TGFB1I1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.