Hck Double Nickase Plasmid (h): sc-401507-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hck Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Hck Double Nickase Plasmid (h) and Hck Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HCK. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Hck Antibody (G-4): sc-166463
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hck Double Nickase Plasmid (h) | sc-401507-NIC | 20 µg | $410.00 | |||
Hck Double Nickase Plasmid (h2) | sc-401507-NIC-2 | 20 µg | $410.00 |
HCK encodes Hck, a Src family non-receptor tyrosine kinase enriched in hematopoietic and myeloid lineages that couples immunoreceptor and cytokine cues to phosphorylation-dependent signaling. Hck participates in pathways controlling innate immune activation, Fc receptor signaling, integrin-mediated adhesion, phagocytosis, and cytoskeletal remodeling, influencing cell migration and inflammatory responses. Through cross-talk with PI3K/AKT, MAPK, and NF-κB-associated networks, Hck helps regulate proliferation, survival, and differentiation in immune cells. Dysregulated HCK activity or expression has been linked to aberrant myeloid signaling and has been studied in contexts including leukemia biology and tumor-associated macrophage function within inflammatory microenvironments.
Hck Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HCK locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HCK. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HCK function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HCK-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.