H2BFWT CRISPR/Cas9 KO Plasmid (h): sc-407111
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- H2BFWT CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the H2BFWT genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
H2BFWT CRISPR/Cas9 KO Plasmid (h) | sc-407111 | 20 µg | $397.00 |
Overview
H2BFWT encodes a human histone H2B family member that participates in nucleosome organization and higher-order chromatin architecture, thereby influencing genome accessibility for transcription, replication, and DNA repair. As a core chromatin component, H2BFWT helps coordinate epigenetic regulation and chromatin-dependent processes such as cell-cycle progression and maintenance of genomic stability. Altered histone dosage or chromatin composition is frequently linked to dysregulated gene expression programs and may modulate pathways implicated in oncogenesis and developmental abnormalities. Consequently, H2BFWT is relevant for studies of chromatin dynamics, transcriptional control, and DNA damage responses in human cells.
H2BFWT CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the H2BFWT gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the H2BFWT together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the H2BFWT open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish H2BFWT protein expression.
This CRISPR knockout system enables efficient generation of H2BFWT-deficient cell models for investigation of H2BFWT signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting H2BFWT exon(s) critical for H2BFWT function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple H2BFWT genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by H2BFWT CRISPR/Cas9 KO Plasmid (h) and H2BFWT CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the H2BFWT locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by H2BFWT HDR Plasmid (h) and H2BFWT HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by H2BFWT homology arms to support homology-directed repair at defined H2BFWT target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.