Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GSTA1 Double Nickase Plasmid (h): sc-403681-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GSTA1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GSTA1 Double Nickase Plasmid (h) and GSTA1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GSTA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GSTA1 Antibody (R-14): sc-100546
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GSTA1 Double Nickase Plasmid (h) | sc-403681-NIC | 20 µg | $410.00 | |||
GSTA1 Double Nickase Plasmid (h2) | sc-403681-NIC-2 | 20 µg | $410.00 |
Glutathione S-transferase alpha 1 (GSTA1) is a cytosolic phase II detoxification enzyme that catalyzes glutathione conjugation to electrophilic xenobiotics and endogenous lipid peroxidation products, facilitating their inactivation and clearance. It contributes to redox homeostasis and protection from oxidative stress by limiting reactive aldehyde accumulation and modulating cellular responses to electrophile-induced damage. GSTA1 activity interfaces with glutathione metabolism and broader antioxidant programs, including pathways coordinated by NRF2/KEAP1 signaling. Altered GSTA1 expression or function has been associated with interindividual variability in drug metabolism and susceptibility to toxicant-induced liver injury, supporting its relevance in pharmacogenomics and hepatocellular stress models.
GSTA1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GSTA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GSTA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GSTA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GSTA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.